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    《亚洲药物制剂科学(英文版)》 2017-01
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    Development of RP-HPLC method for simultaneous determination of docetaxel and curcumin in rat plasma: Validation and stability

    Dong Wuk Kim;Abid Mehmood Yousaf;Dong Xun Li;Jong Oh Kim;Chul Soon Yong;Kwan Hyung Cho;Han-Gon Choi;College of Pharmacy & Institute of Pharmaceutical Science and Technology, Hanyang University;Faculty of Pharmacy, University of Central Punjab;National Pharmaceutical Engineering Centre for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine;College of Pharmacy, Yeungnam University;College of Pharmacy, Inje University;  
    The purpose of the present research was to develop a suitable, simple, precise, accurate,robust, and reproducible RP-HPLC method for a reliable simultaneous quantification of docetaxel(DTX) and curcumin(CCM) in rat plasma samples using paclitaxel(PTX) as an internal standard. The samples were assayed by the Agilent 1260 Infinity HPLC instrument using a Capcell Pak C_8 column(4.6 mm × 150 mm, 5 μm) under isocratic conditions. The mobile phase consisted of acetonitrile and triple distilled water(40/60, v/v) with a flow rate of 1.0 ml/min. The eluent was monitored at 230 nm for simultaneous measurement of curcumin and docetaxel. The method was validated by determining system suitability, selectivity, sensitivity, linearity, inter-day and intra-day precision, accuracy, robustness, and stability in accordance with the guidelines of the United States Food and Drug Administration(FDA).The developed chromatographic method proved to be simple, precise, accurate, robust and reproducible. Moreover, the samples showed stability at room temperature over a period of 48 h. Thus, this method would be employed for routine simultaneous quantification of docetaxel and curcumin in rat plasma samples.
    【Fund】: supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science ICT and Future Planning (NRF-2015R1C1A1A01051698)
    【CateGory Index】: R965
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