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    《Chinese Journal of Critical Care Medicine》 2019-06
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    Expression of NLRP3 inflammasome in renal tissue of rats with acute kidney injury induced by ischemia-reperfusion and intervention of dexamethasone

    Xuan Xian-fa;Wang Yu-xin;Zhang Yi-qin;Zhou Li-na;Wu Ting;Zhang Si-yu;Zhao Ben-hua;Department of Nephrology,the Second Affiliated Hospital of Xiamen Medical College(the Second Hospital of Xiamen);  
    Objective To investigate the expression of NLRP3 inflammasome and the effect of dexamethasone in renal tissue of rats with acute kidney injury induced by ischemia-reperfusion.Methods Twenty-one normal male SD rats were randomly divided into three groups,respectively ischemia-reperfusion group(Group A),sham operation group(Group B)and dexamethasone+ischemia reperfusion group(Group C).The differences of serum creatinine,urea nitrogen and NLRP3 expression among the groups were compared.The effect of dexamethasone on the content of NLRP3 inflammatory body in rats was analyzed.The expression of NLRP3 protein in rat renal tissue was detected by Western blot; the levels of IL-10 and IL-18 in rat kidney were detected by ELISA; the expression of NLRP3 in kidney was detected by immunohistochemistry,renal tubulointerstitial injury was detected by pathological examination.Results The levels of serum creatinine,urea nitrogen,IL-1β,IL-18 and NLRP3 inflammasome protein in group A rats were significantly higher than those in group B[(89.5±10.8)μmol/L(22.3±3.1)μmol/L,(23.1 ±5.3)mmol/L(4.8 ±0.7)mmol/L,(0.39±0.11)(0.08 ±0.01),(21.7 ±5.6)pg/mL(5.7±1.9)pg/mL,(42.5 ±6.7)pg/mL (11.4 ±2.3)pg/mL; P0.05].The levels of serum creatinine,urea nitrogen,IL-1β,IL-18 and NLRP3 inflammasome protein in group C were significantly lower than those in group A[(53.4±8.3)μmol/L(89.5 ±10.8)μmol/L,(9.6 ±2.9)mmol/L(23.1 ±5.3)mmol/L,(0.21 ±0.07)(0.39 ±0.11); P0.05].Immunohistochemistry showed that the expression of NLRP3 in renal tubular epithelial cells in rats with acute kidney injury after ischemia-reperfusion was significantly increased.Pathological examination showed that the tubulointerstitial injury was severe in the ischemia-reperfusion group.Conclusion Acute kidney injury after ischemia-reperfusion is associated with an increase in serum creatinine,IL-1β,IL-18 and NLRP3 inflammasome levels and NLRP3 expression of renal tissue.Intervention with dexamethasone can reduce the NLRP3 inflammasome content in renal tissue and reduce the degree of damage to renal tissue.
    【Fund】: 厦门市科技局2016科技惠民项目(3502220164060)
    【CateGory Index】: R692
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    【Citations】
    Chinese Journal Full-text Database 4 Hits
    1 Ding Lihong;Liu Bicheng;Chen Pingsheng;Gong Yuxiang;Yang Minyu;Institute of Nephrology,Zhongda Hospital,Southeast University;;Influence of albumin on expression of NLRP3 inflammasome in renal tubular epithelial cells[J];中华肾脏病杂志;2015-10
    2 NIE Han;SU Ke;LONG Yan;JIANG Shanshan;SUN Qing;Department of endcrinology, Affiliated Hospital of Guilin Medical College;;Effect of artesunate on expressions of Toll-like receptor 4 and interleukin-8 in renal tissues of diabetic nephropathy rats[J];天津医药;2015-04
    3 Zhou Li-na;Wang Yu-xin;Fang Lin;Wu Ting;Yu Yi;Department of Nephrology, Second Hospital of Xiamen City;Department of Urinary Surgery, Zhongshan Hospital of Xiamen University;Department of Hemopurification,Fuzhou General Hospital of Nanjing Military Command of Chinese PLA;;Endothelial progenitor cells repair ischemia-reperfusion injury in rats[J];中国组织工程研究;2014-32
    4 ZHUANG Mei,WU Dai-qin,LEI Da-wei (Department of Cardiology,Affiliated Hospital of Guiyang Medical College, Guiyang 550004,Guizhou Province,China);Effect of different dexamethasone doses on apoptosis of myocardiocytes and expression of NF-кB in rats following ischemia/reperfusion[J];中华老年心脑血管病杂志;2012-05
    【Co-citations】
    Chinese Journal Full-text Database 1 Hits
    1 Xuan Xian-fa;Wang Yu-xin;Zhang Yi-qin;Zhou Li-na;Wu Ting;Zhang Si-yu;Zhao Ben-hua;Department of Nephrology,the Second Affiliated Hospital of Xiamen Medical College(the Second Hospital of Xiamen);;Expression of NLRP3 inflammasome in renal tissue of rats with acute kidney injury induced by ischemia-reperfusion and intervention of dexamethasone[J];中国急救医学;2019-06
    【Secondary Citations】
    Chinese Journal Full-text Database 10 Hits
    1 JIANG Shanshan;LONG Yan;SU Ke;NIE Han;HUANG Lili;YANG Fan;LI Zhengming;XUN Jingqiong;Department of Endocrinology, Affiliated Hospital of Guilin Medical College;;Effects of artesunate on high glucose-induced cell apoptosis, TNF-α and IL-8 expression in renal tubular epithelial cells[J];天津医药;2015-01
    2 HUANG Long;LIAO Ting-ting;GONG Jun;LUO Wei-feng;Department of Nephrology,the First Affiliated Hospital of Liuzhou Medical College in the Guangxi Zhuang Autonomous Region;;The influence of Bailing capsule on microinflammatory state in diabetic nephropathy with chronic renal failure[J];中国当代医药;2014-15
    3 LIU Liang;ZUO Lian-Fu;WANG Jing;Department of FCM Analysis,Tumor Institute,The Fourth Hospital of Hebei Medical University;;Effects of artesunate on membrane potential of mitochondria and cell apoptosis of Ec9706 cells[J];解放军医学杂志;2014-01
    4 LI He-wen;WANG Jia-feng;YU Guang;Department of Nephrology,Changhai Hospital,Second Military Medical University;Department of Anesthesiology,Changhai Hospital,Second Military Medical University;;Role of microRNAs in renal ischemia-reperfusion injury:an update[J];第二军医大学学报;2013-12
    5 ZHANG Xiao-dong;FANG Jing-ai;LI Xiu-xiu;SUN Yan-yan;LIU Wen-yuan;CHANG Qin-tao;Renal Division,First Hospital of Shanxi Medical University;;Effect of Yishen capsule on tubulointerstitial expression of TLR4 and NF-κB in experimental diabetic nephropathy[J];中华肾病研究电子杂志;2013-04
    6 LIU Hao,WU Ran,JIA Rui-peng,ZHONG Bing,YU Peng, ZHAO Yan.Department of Urology,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,China;Impact of ischemic preconditioning on dynamics of homing of endothelial progenitor cells after renal ischemia reperfusion injury[J];中华肾脏病杂志;2013-03
    7 CHEN Bo1,BO Cheng-jia2,JIA Rui-peng2,ZHU Jia-geng2, LI Wen-cheng2,WU Ran2,LIU Hao2,GE Yu-zheng2(1.School of Medicine,Southeast University,Nanjing 210009,China;2.Department of Urology, Nanjing First Hospital Affiliated to Nanjing Medical University,Nanjing 210006,China);Anti-apoptosis role of endothelial progenitor cells in the eraly phase of acute renal ischemia reperfusion injury[J];东南大学学报(医学版);2012-05
    8 YANG Mengxue1,2,GAN Hua1,SHEN Qing1 (1.Department of Nephrology,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016; 2.Department of Endocrinology,First Affiliated Hospital of Zunyi Medical College,Zunyi Guizhou 563003,China);Effect of LPS on the level of TLR4 and on the expression of NF-κB and Notch1 in monocytes from patients with type 2 diabetic nephropathy[J];中南大学学报(医学版);2012-06
    9 HUANG Fa-jun,ZHAN Guang-jie,ZHANG Hong Department of Biochemistry,Medical College of Hubei Institute for Nationalities,Enshi 445000,China;Effects of artesunate on the expression level of TLR-4 and TGF-β with asthma rats[J];中国医药生物技术;2011-04
    10 MA Xing-yi,WU Wei-hua,ZENG Yan,et al.(Department of Nephrology,West China Hospital of Sichuan University,Chengdu 610041,China);The anti-inflammatory effect and its mechanism of Artemisia on the renal of UUO rats[J];重庆医学;2010-12
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