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    《Chinese Journal of Critical Care Medicine》 2019-02
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    Effect of dexmedetomidine on endoplasmic reticulum stress in lung tissue of rats with ventilation-associated lung injury and its relationship with PI3K/Akt signaling pathway

    Qu Min;Mao Shun-hong;Jiao Bao-jie;Liu Xiang-ge;Yang Qiang;Shi Dan-dan;Wang Ya-li;Ma Zhi-hong;Li Hui-zhi;The Second Department of Anesthesiology,Cangzhou Center Hospital;  
    Objective To investigate the effects of dexmedetomidine on endoplasmic reticulum stress-induced apoptosis and PI3 K/Akt signaling pathway in rats with ventilation-associated lung injury. Methods Eighty Sprague-Dawly(SD) rats were randomly(random number) divided into 4 groups(n = 20 each) : the control group( Group C), Mechanical ventilation Group( Group V),Dexmedetomidine groups(Group D) and Dexmedetomidine +LY294002 group(group DL). Group C,no mechanical ventilation, and breathed independently; Mechanical ventilation in group V, D and DL for4 h, dexmedetomidine 5. 0 μg/( kg · h) were infused intravenously, 20 min before mechanical ventilation, 5.0 μg/(kg · h) intravenous infusion during mechanical ventilation. In the Group DL,LY294002 0. 3 mg/kg was infused intravenously 5 min prior to dexmedetomidine. Group V was given normal saline. Rats were sacrificed at 4 hours after mechanical ventilation and the lung permeability index(LPI) and lung wet/dry weight(W/D) ratio were measured. Western blot was used to detect the level of Akt, phosphorylated Akt(P-Akt), glucose-regulated protein 78(GRP78), CAAT enhancer binding protein homologous protein(CHOP), and caspase-12. The pathological changes of lung tissues were observed under light microscope, and the alveolar injury rate(IAR) was determined. The lung cell apoptosis was observed and apoptosis index was calculated by TUNEL method. Results Compared with the group C, ratio of W/D, LPI, IAR(%), AI(%) in group V and group DL increased, and the expressions of GRP78, CHOP, caspase-12 increased(group C: 0.35 ±0.02,0.28 ± 0.02,0.51 ±0.03; group V: 1.27 ± 0. 11,1. 09 ± 0. 13,1. 37 ± 0. 15; group DL: 0. 97 ± 0.08,0.66 ± 0. 07,1. 01 ± 0. 09; P 0. 05),the expression of P-Akt decreased( group C: 0. 81 ± 0. 04; group V:0.23 ±0.01; group DL:0.44 ±0.02;P 0.05). Compared with the group V,group D and group DL Ratio of W/D, LPI, IAR(%), AI(%) decreased, and the expressions of GRP78,CHOP, caspase-12 decreased( group V: 1.27 ±0.11, 1.09 ±0.13,1. 37 ±0.15; group DL: 0. 97 ±0.08,0. 66 ±0.07,1.01 ± 0.09; group D : 0.62 ± 0.04, 0.43 ± 0.05,0.79 ± 0.07; P 0.05), the expression of P-Akt increased(group V: 0. 23 ± 0. 01; group DL: 0. 44 ± 0. 02; group D: 0. 65 ±0.03; P 0. 05),the pathological changes of lung tissues were significantly attenuated; Compared with the group D, ratio of W/D, LPI, IAR( %), AI(%) in group DL increased, and the expressions of GRP78, CHOP,caspase-12 increased( group D: 0.62 ±0.04,0.43 ±0. 05,0. 79 ±0. 07; group DL: 0. 97 ±0.08,0.66 ± 0.07,1.01 ± 0.09;P 0.05),the expression of P-Akt decreased(group D: 0. 65 ± 0. 03;group DL: 0.44±0.02; P0.05),and the lung tissue pathological damage was heavier. Conclusion Dextomidine can alleviate ventilation-associated lung injury in rats, which is related to activation of PI3 K/Akt signaling pathway, inhibition of endoplasmic reticulum stress, and reduction of apoptosis in lung tissue.
    【CateGory Index】: R614
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    【Citations】
    Chinese Journal Full-text Database 4 Hits
    1 Qu Min;Mao Shun-hong;Jiao Bao-jie;Liu Xiang-ge;Yang Qiang;Shi Dan-dan;Ma Zhi-hong;Li Hui-zhi;The Second Department of Anesthesiology,Cangzhou Center Hospital;;Effects of dexmedetomidine on cell apoptosis in lung tissue in a rat model of ventilator-induced lung injury[J];中国急救医学;2018-03
    2 Qu Min;Jiao Baojie;Mao Shunhong;Yao Zhongyan;Ma Zhihong;Li Huizhi;Yang Qiang;Liu Xiangge;Department of Anesthesiology,Cangzhou Center Hospital;;Effect of dexmedetomidine on expression of aquaporin 5 in lung tissues in a rat model of ventilator-induced lung injury[J];中华麻醉学杂志;2016-10
    3 Yao Xiangyan;Meng Fanmin;Zhang Jiaqiang;Du Xianhui;Department of Anesthesiology,He'nan Provincial People's Hospital;;Effects of dexmedetomidine pretreatment on cell apoptosis induced by endoplasmic reticulum stress and c-Jun N-terminal kinase pathway during one-lung ventilation in rats[J];中华麻醉学杂志;2015-01
    4 LIU You-ping~1,YAN Dong-mei~1,CHEN Chuan-ning~1,DUAN Chun-yan~1,CHEN Shao-kun~2,LI Hong~1,DAI Rong-yang~1 (1 Department of Biochemistry,2 Department of Biology and Genetics,Luzhou Medical College,Luzhou 646000,China.;PI3K/Akt regulates endoplasmic reticulum stress-mediated GRP78 induction[J];中国病理生理杂志;2011-04
    【Co-citations】
    Chinese Journal Full-text Database 10 Hits
    1 Yang Qiang;Qu Min;The Second Department of Anesthesiology, Cangzhou Center Hospital;;Study on the pulmonary protective effect of dexmedetomidine in ophthalmic surgery[J];国际呼吸杂志;2019-06
    2 Qu Min;Mao Shun-hong;Jiao Bao-jie;Liu Xiang-ge;Yang Qiang;Shi Dan-dan;Wang Ya-li;Ma Zhi-hong;Li Hui-zhi;The Second Department of Anesthesiology,Cangzhou Center Hospital;;Effect of dexmedetomidine on endoplasmic reticulum stress in lung tissue of rats with ventilation-associated lung injury and its relationship with PI3K/Akt signaling pathway[J];中国急救医学;2019-02
    3 Yang Qiang;Qu Min;Mao Shunhong;Jiao Baojie;Liu Xiangge;Shi Dandan;Wang Yali;Department of Anesthesiology,Cangzhou Center Hospital;Department of Obstetrics,Cangzhou Center Hospital;;Effect of methylprednisolone on endoplasmic reticulum stress in rats with ventilator-induced lung injury:the relationship with PI3K/Akt signaling pathway[J];中华麻醉学杂志;2018-08
    4 Qu Min;Mao Shun-hong;Jiao Bao-jie;Liu Xiang-ge;Yang Qiang;Shi Dan-dan;Ma Zhi-hong;Li Hui-zhi;The Second Department of Anesthesiology,Cangzhou Center Hospital;;Effects of dexmedetomidine on cell apoptosis in lung tissue in a rat model of ventilator-induced lung injury[J];中国急救医学;2018-03
    5 Liu Xiaole;Zhou Junhui;Fang Fang;Meng Rui;Xi Gaoyuan;Meng Xianhui;Department of Anesthesiology,Henan Provincial Chest Hospital;;Effect of penehyclidine hydrochloride on non-ventilated lung injury and endoplasmic reticulum stress in patients with radical resection of pulmonary cancer[J];中国医药;2017-11
    6 Wang Liangrong;Xiong Xiangqing;Yang Jianping;Department of Anesthesiology,First Affiliated Hospital,Soochow University;Department of Anesthesiology,First Affiliated Hospital of Wenzhou Medical University;;Effects of FTY720 pretreatment on lung injury induced by limb ischemia-reperfusion in rats[J];中华麻醉学杂志;2016-12
    7 Xia Li;Zhang Yunlong;Yang Pengju;Dong Tieli;Department of Anesthesia,Second Affiliated Hospital of Zhengzhou University;Department of Anesthesia,Women & Infants Hospital of Zhengzhou;;Effect of curcumin pretreatment on JNK signaling pathway during one-lung ventilation-induced acute lung injury in mice[J];中华麻醉学杂志;2016-07
    8 Zhou Bin;Xiao Fan;Huang Dan;Hua Fuzhou;Xu Guohai;Luo Zhenzhong;Department of Anesthesiology,Second Affiliated Hospital,Nanchang University;;Role of JNK signaling pathway in dexmedetomidine-induced reduction of spinal neurotoxicity induced by lidocaine in rats[J];中华麻醉学杂志;2015-10
    9 CHEN Dan;SONG Dong;YE Yu-zhu;HE Jin-bo;CHEN Lei;QIU Xiao-xiao;LIN Li-na;WANG Wan-tie;Institute on Ischemia/Reperfusion Injury,The First Affiliated Hospital,Wenzhou Medical University;Department of Anesthesiology,The First Affiliated Hospital,Wenzhou Medical University;;Dexmedetomidine alleviates lung ischemia-reperfusion injury through CHOP pathway in mice[J];中国病理生理杂志;2015-06
    10 Deng Xiangqun;Peng Bo;Department of Histology and Embryology,Shaoyang Medical College;Department of Occupational Hazardous Control,Center for Disease Control and Prevention;;Expressions of TNFAIP3 and GRP78 gene in radiotherapy of nasopharyngeal carcinoma[J];现代肿瘤医学;2015-05
    【Secondary Citations】
    Chinese Journal Full-text Database 7 Hits
    1 Qu Min;Jiao Baojie;Mao Shunhong;Yao Zhongyan;Ma Zhihong;Li Huizhi;Yang Qiang;Liu Xiangge;Department of Anesthesiology,Cangzhou Center Hospital;;Effect of dexmedetomidine on expression of aquaporin 5 in lung tissues in a rat model of ventilator-induced lung injury[J];中华麻醉学杂志;2016-10
    2 Yuan-xu JIANG;Zhong-liang DAI;Xue-ping ZHANG;Wei ZHAO;Qiang HUANG;Li-kun GAO;Department of Anesthesiology,Shenzhen People's Hospital;Department of Anesthesiology,Zhujiang Hospital,Nanfang Medical University;Department of Pathology,Shenzhen People's Hospital;;Dexmedetomidine Alleviates Pulmonary Edema by Upregulating AQP1 and AQP5 Expression in Rats with Acute Lung Injury Induced by Lipopolysaccharide[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2015-05
    3 CHEN Peng1,YANG Cheng-ming1,ZENG Chun-yu1,WANG Xu-kai1,XIONG Xiu-qin2,HE Duo-fen1 (1Department of Cardiology,2Department of Ultrasound Diagnosis,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China.);Effect of endoplasmic reticulum stress on cardiac myocyte apoptosis in mouse congestive heart failure induced by myocardial infraction[J];中国病理生理杂志;2010-06
    4 HAO Xiao-yan,BIAN Yun-fei,LI Mao-lian,GAO Fen,YANG Hui-yu,ZHENG Jie,XIAO Chuan-shi(Department of Cardiology,The Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,China.);Effects of adiponectin on reducing endoplasmic reticulum stress injury induced by hypoxia-reoxygenation in cultured rat neonatal cardiomyocytes[J];中国病理生理杂志;2010-06
    5 GAO Jun-peng1,2,CAI Ding-fang1,2(1Zhongshan Hospital,2Unit for Neurology,Department of Integrative Medicine,Fudan University,Shanghai 200032,China.);Endoplasmic reticulum stress mediates the neurotoxic effect of misfolded proteins in neurodegenerative diseases[J];中国病理生理杂志;2008-01
    6 @CHEN Chang$Department of Anesthesiology,Zhongnan Hospital,Wuhan University!Wuhan 430071,China @ZHANG Zong-ze$Department of Anesthesiology,Zhongnan Hospital,Wuhan University!Wuhan 430071,China @CHEN Kai$Department of Anesthesiology,Zhongnan Hospital,Wuhan University!Wuhan 430071,China @GUO Wei$Department of Anesthesiology,Zhongnan Hospital,Wuhan University!Wuhan 430071,China @PENG Mian$Department of Anesthesiology,Zhongnan Hospital,Wuhan University!Wuhan 430071,China @WANG Yan-lin$Department of Anesthesiology,Zhongnan Hospital,Wuhan University!Wuhan 430071,China;Effect of dexmedetomidine on NF-κB activity during ventilator-induced lung injury in dogs[J];中华麻醉学杂志;2013-10
    7 @ZHOU Jun-hui$Department of Anesthesiology,the First Affiliated Hospital of Wenzhou Medical University!Wenzhou 325035,China @WANG Liang-rong$Department of Anesthesiology,the First Affiliated Hospital of Wenzhou Medical University!Wenzhou 325035,China @HAO Mao-lin @YING Lei @SUN Qin @WANG Wan-tie;Effects of targeted small interfering RNA on pneumocyte apoptosis and CCAAT/enhancer-binding protein homologous protein in pulmonary ischemia/reperfusion injury in mice[J];中华实验外科杂志;2013-08
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